Open AccessProspective Isolation of Bronchiolar Stem Cells Based Upon Immunophenotypic and Autofluorescence Characteristics
Author(s) -
Teisanu Roxana M.,
Lagasse Eric,
Whitesides John F.,
Stripp Barry R.
Publication year - 2009
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1634/stemcells.2008-0838
Subject(s) - biology , stem cell , progenitor cell , cd34 , adult stem cell , stem cell marker , microbiology and biotechnology , pathology , endothelial stem cell , in vitro , genetics , medicine
Bronchiolar stem cells have been functionally defined in vivo on the basis of their resistance to chemical (naphthalene) injury, their infrequent proliferation relative to other progenitor cell types, and their coexpression of the airway and alveolar secretory cell markers Clara cell secretory protein and pro‐surfactant protein C, respectively. Cell surface markers that have previously been used for their prospective isolation included Sca‐1 and CD34. Using transgenic animal models associated with stem cell expansion, ablation, and lineage tracing, we demonstrate that CD34 pos cells do not belong to the airway epithelial lineage and that cell surface Sca‐1 immunoreactivity does not distinguish between bronchiolar stem and facultative transit‐amplifying (Clara) cell populations. Furthermore, we show that high autofluorescence (AF high ) is a distinguishing characteristic of Clara cells allowing for the fractionation of AF low bronchiolar stem cells. On the basis of these data we show that the defining phenotype of the bronchiolar stem cell is CD45 neg CD31 neg CD34 neg Sca‐l low AF low . This refinement in the definition of bronchiolar stem cells provides a critical tool by which to assess functional and molecular distinctions between bronchiolar stem cells and the more abundant pool of facultative transit‐amplifying (Clara) cells. S TEM C ELLS 2009;27:612–622