Open AccessIn Vivo Osteoprogenitor Potency of Human Stromal Cells from Different Tissues Does Not Correlate with Expression of POU5F1 or Its Pseudogenes
Author(s) -
Kaltz Nikolas,
Funari Alessia,
Hippauf Sandra,
Delorme Bruno,
Noël Danièle,
Riminucci Mara,
Jacobs Volker R.,
Häupl Thomas,
Jorgensen Christian,
Charbord Pierre,
Peschel Christian,
Bianco Paolo,
Oostendorp Robert A.J.
Publication year - 2008
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1634/stemcells.2008-0304
Subject(s) - biology , stem cell , mesenchymal stem cell , stromal cell , pseudogene , microbiology and biotechnology , haematopoiesis , bone marrow , immunology , cancer research , genetics , gene , genome
Expression of “stemness” markers is widely used as a predictor of stem cell properties of mesenchymal stem cells (MSC). Here, we show that bone marrow‐derived (BM)‐MSC show stem cell‐like behavior in vivo; that is, they form ossicles with formation of bone, formation of adipocytes, and establishment of the murine hematopoietic microenvironment. Multipotent umbilical vein‐derived stromal cells (UVSC), on the other hand, do not form bone, nor do they give rise to adipocytes in vivo. Despite these differences in stem‐cell‐like behavior, BM‐MSC and UVSC express the two transcripts variants of POU5F1 at a similar level. Also, we found that in BM‐MSC and UVSC, POU5F1 is detectable. However, more than 89% of the POU5F1 transcripts correspond to the POU5F1P1, ‐P3, or ‐P4 pseudogene. Despite low‐level expression of POU5F1, we were unable to precipitate POU5F1 protein in either BM‐MSC or UVSC. These results demonstrate that MSC stemness does not correlate to expression of POU5F1 transcripts or its pseudogenes. Disclosure of potential conflicts of interest is found at the end of this article.