Open AccessEvaluation of Sca‐1 and c‐Kit As Selective Markers for Muscle Remodelling by Nonhemopoietic Bone Marrow Cells
Author(s) -
Wong Sharon H.A.,
Lowes Kym N.,
Bertoncello Ivan,
Quigley Anita F.,
Simmons Paul J.,
Cook Mark J.,
Kornberg Andrew J.,
Kapsa Robert M.I.
Publication year - 2007
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1634/stemcells.2006-0194
Subject(s) - biology , mesenchymal stem cell , bone marrow , population , selectable marker , stem cell , stem cell marker , skeletal muscle , cell , microbiology and biotechnology , immunology , genetics , anatomy , gene , transgene , demography , sociology
Bone marrow (BM)‐derived cells (BMCs) have demonstrated a myogenic tissue remodeling capacity. However, because the myoremodeling is limited to approximately 1%–3% of recipient muscle fibers in vivo, there is disagreement regarding the clinical relevance of BM for therapeutic application in myodegenerative conditions. This study sought to determine whether rare selectable cell surface markers (in particular, c‐Kit) could be used to identify a BMC population with enhanced myoremodeling capacity. Dystrophic mdx muscle remodeling has been achieved using BMCs sorted by expression of stem cell antigen‐1 (Sca‐1). The inference that Sca‐1 is also a selectable marker associated with myoremodeling capacity by muscle‐derived cells prompted this study of relative myoremodeling contributions from BMCs (compared with muscle cells) on the basis of expression or absence of Sca‐1. We show that myoremodeling activity does not differ in cells sorted solely on the basis of Sca‐1 from either muscle or BM. In addition, further fractionation of BM to a more mesenchymal‐like cell population with lineage markers and CD45 subsequently revealed a stronger selectability of myoremodeling capacity with c‐Kit/Sca‐1 ( p < .005) than with Sca‐1 alone. These results suggest that c‐Kit may provide a useful selectable marker that facilitates selection of cells with an augmented myoremodeling capacity derived from BM and possibly from other nonmuscle tissues. In turn, this may provide a new methodology for rapid isolation of myoremodeling capacities from muscle and nonmuscle tissues. Disclosure of potential conflicts of interest is found at the end of this article.