Open AccessExpression of Adhesion Molecules on CD34 + Cells in Peripheral Blood of Non‐Hodgkin's Lymphoma Patients Mobilized with Different Growth Factors
Author(s) -
Gazitt Yair,
Shaughnessy Paul,
Liu Qun
Publication year - 2001
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1634/stemcells.19-2-134
Subject(s) - cd34 , homing (biology) , biology , lymphoma , bone marrow , stem cell , cell adhesion molecule , microbiology and biotechnology , immunology , andrology , medicine , ecology
Adhesion molecules on CD34 + cells were implicated in the process of peripheral blood stem cell (PBSC) mobilization and homing. We studied the mobilization of CD34 + Thy1 + cells, CD34 + very late‐acting antigen (VLA)4 + cells, and CD34 + L‐selectin + cells in non‐Hodgkin's lymphoma patients mobilized with cyclophosphamide plus G‐CSF, GM‐CSF, or GM‐CSF followed by G‐CSF. The mean percentage of CD34 + cells in the bone marrow (BM) expressing Thy1 was 23.6% ± 11% and 17.8% ± 8% in the PB before mobilization, and was markedly decreased to 4.5% ± 3.3% in the apheresis collections. Similarly, the mean percentage of CD34 + cells expressing L‐selectin was 35.8% ± 4.3% in the BM, 21.6% ± 4.1% in the PB before mobilization and was markedly decreased to 9.1% ± 2.5% in the apheresis collections. Patients in the three arms of the study had a similar pattern of CD34 + Thy1 + and CD34 + L‐selectin + cell mobilization. Also, a similar pattern of coexpression of CD34 + Thy1 + and CD34 + L‐selectin + cells was observed when the patients were regrouped as “good mobilizers” (≥2 × 10 6 CD34 + CD45 dim cells/kg, in four collections) and “poor mobilizers” (<0.4 × 10 6 CD34 + CD45 dim cells/kg, in two collections). The mean percentage of CD34 + cells expressing VLA‐4 in the BM and PB was relatively high (73.4% ± 12% and 65.4% ± 6.6%, respectively) and dropped considerably in the PBSC collections to 43.5% ± 7.1% with a similar pattern observed for patients in arms A, B, and C. However, when the patients were regrouped as “good mobilizers” and “poor mobilizers,” a higher percentage of CD34 + cells expressing VLA‐4 was observed in the PBSC of the pooled “good mobilizers” (50.5% ± 9% versus 36.3% ± 6.4%; p = 0.01). We conclude that release of CD34 + cells to the PB involves a general downregulation of Thy1, L‐selectin and VLA‐4 on CD34 + cells, irrespective of the growth factor used for mobilization. However, good mobilizers had a relatively higher percentage of CD34 + cells expressing the VLA‐4 antigen.