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A new method for the quantitative determination of free triiodothyronine (fT3) and an accurate method for the quantitative determination of free thyroxine (fT4) in human plasma were developed. The percentages of T3 and T4 in plasma were studied by thin-layer chromato-graphy. An elutable fraction (EF) of T3, ratio of free 125I-T3 to protein-bound 125I-T3, was obtained using Sephadex-microcolumn chromatography. EF of T4 was obtained by the same method. Mean normal total T3 (tT3) and total T4 (tT4), calculated from plasma butanol-extractable iodine (BET) and molar percentages of T3 and T4, were 0. 63 and 5. 59mg per 100ml plasma, respectively. Mean normal fT3 and fT4, calculated from tT3, tT4 and EF values of T3 and T4, were 2.43 and 2.21mμg per 100ml plasma, respectively. The fT3 values can be obtained by the present method more easily and speedily than by the method of Nauman et al. The present fT4 values, in consideration of BET and the percentage of T4 in plasma, represent a more accurate one than the earlier data by Lee et al.

Quantitative Determination of Total and Free Triiodothyronine and Thyroxine