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The fundamental physical phenomena involved in the application of fiber optics to in vivo densitometry or oximetry were investigated on bovine blood, and a new method for calibrating dye-dilution curve was proposed. 1) Reflected light was increased by the movement of blood when fiber optics were positioned either across or against blood flow, and decreased when they were positioned along the blood flow. The increment or decrement of reflected light attained its maximum with a flow rate of 10cm/sec, and reflected light came to the original level at rest with further increase in flow rate. The orientation of discoid red blood cells, transformation of the cells, and the occurrence of turbulence were taken into consideration as possible causes. 2) Reflected light was decreased with admixture of dye. The ratio of this decrement to reflected light before admixture of dye did not show any sample-to-sample variation. 3) This relationship was utilized for calibrating dye-dilution curves. Cardiac output calculated by this method agreed well with that by the conventional cuvette method.

Studies on Light Reflection of Blood in the Application of Fiber Optics for Dye-dilution Curve