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Urine analysis of patients with progressive muscular dystrophy (PMD) revealed that one of these patients excreted a considerable amount of diphos-phopy ridine nucleotide (DPN) in urine. DPN in urine was identified by column chromatography using Dowex 1×10 (formate type), paper chromato-graphy, absorption spectrum, and by absorbency changes after being reacted with potassium cyanide and with yeast alcohol dehydrogenase. DPN excretion in urine in some other PMD eases was evidenced by the alcohol dehydrogenase method. Numerous urine specimens from normal and diseased individuals were also examined for DPN, but urinary DPN could be demonstrated in no case other than those of PMD. The significance of DPN excretion in PMD was discussed in view of the fact that DPN is an important coenzyme in cellular metabolism and that so far no report has appeared on the occurrence of appreciable amounts of DPN in human urine.

Occasional Appearance of Diphosphopyridine Nucleotide in Urine of Patients with Progressive Muscular Dystrophy