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I. The lumbar route operation, described in the foregoing paper, was performed on dogs, the operation field of which was previously deafferented, either prone fastened on the table or freely sitting or standing there. As a matter of course narcosis was never resorted to. Making use of these dogs the bloods were collected from the suprarenal gland during the fastening period and the free interval alternately, and compared each other. In half of the cases the fastening down induced an evident increase in the output rate of epinephrine, though the degree of acceleration was somewhat inferior to that in the sensory stimulation experiments. In them the violent reactions against fastening, as yelling, struggling, were simultaneously manifest. In the rest the fastening failed to hasten the output velocity of epinephrine. In half of the latter instances the dogs were almost quiet during the confinement. II. Sensory stimulation was also again tried on some dogs in succession to the fastening experimentation. In almost all cases the stimulation of the sensory nerve called forth a faster rate of epinephrine liberation. The rate under stimuli was nearly 2-5 times as fast as that of the rest spells, seldom 8 times. Of the constancy in occurrence of accelerating effect and the ratio of augmentation to the rate during the rest interval the present results achicved with the lumbar route method coincide well with those obtained with the cava pocket method on non-anaesthetized dogs, but the absolute values of the rates during the spells of rest as well as stimuli in the present investigation are far inferior to the previous results with the cava pocket.

Effect of Fastening and of Sensory Stimulation upon the Rate of Epinephrine Output from the Suprarenal Gland in Dogs