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We examined the method of sample digestion and the analytical conditions for simultaneous multi-determination of trace element concentrations by inductively coupled plasma mass spectrometry (ICP-MS) in biological samples. In order to eliminate contaminations by extraneous elements from reagents and instruments, we chose plastic tubes and a Teflon decomposition vessel and super pure grade nitric acid for sample decomposition. Furthermore, standard solutions were prepared by mixing stock solutions of trace elements with a mixture of sample solutions to eliminate matrix interference. Quality control measurements using a standard reference material (SRM bovine liver) showed that the multi-analysis method established in the present study is very accurate and useful for determination of trace element concentrations in biological samples. Using this procedure, we determined the distribution of Zn, Cu, Rb, Mn and Mo in seven discrete regions of the rat brain and Zn and Mn in their synaptosomal fractions.

Simultaneous Multi-Determination of Brain Trace Element Concentrations by Inductively Coupled Plasma Mass Spectrometry.