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Using a modified reverse hemolytic plaque assay, we measured in vitro production of Dermatophagoides farinae allergen-specific IgE and Ig(G + M) antibodies by peripheral blood mononuclear cells in 19 patients with extrinsic bronchial asthma. Circulating the allergen-specific IgE was estimated by means of the Phadebas radioallergosorbent test system. We found a significant difference in the production of the allergen-specific IgE antibody by the cells between asthmatic patients with positive and negative circulating the allergen-specific IgE.

tohoku journal of experimental medicine

A production of Dermatophagoides farinae allergen-specific IgE and Ig(G+M) antibodies by cultured peripheral blood mononuclear cells of patients with extrinsic bronchial asthma.