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To prepare anti-M and -N of a sufficient potency, M or N substance trapped in autologous ghost was intravenously administered to rabbits. The potency and specificity of antisera prepared were compared with antisera prepared by other immunization methods. Rabbits immunized intravenously or intramuscularly with M or N substance alone produced no significant antibodies. Immunization with the substance loading ghost produced crude antisera of relatively low titers, but they were proved to contain specific anti-M and -N after absorption procedure which were higher than those prepared by usual intravenous injection of red cells. There was remarkable difference in immunoglobulin class between antisera prepared with the substance loading ghost and that by red cell immunizations; the former consisted of IgM and IgA, and the latter of IgG and IgA. It was considered that intravenous injection of the antigen trapped in ghost is useful for preparing hemagglutinin of sufficient titers.

A new method to prepare rabbit immune anti-M and -N sera using blood group substance trapped in autologous red cell ghost as an immunogen.