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The effect of heparin Na on the actions of various bronchoconstrictors was examined. Guinea pig tracheal strips were suspended in bioassay glass chambers and superfused with Krebs-Henseleit solution, pH 7.4, at 37 degrees C saturated with oxygen and carbon dioxide gas (95:5, v/v). Contraction of the tissue was detected by an isotonic transducer and displayed on a polyrecorder. Heparin Na attenuated histamine-, prostaglandin F2 alpha-, acetylcholine-, serotonin-induced contractile responses dose-dependently, and shifted the dose-response curves of histamine, prostaglandin F2 alpha, acetylcholine, serotonin and bradykinin downward.

Effect of heparin Na on the action of various bronchoconstrictors in guinea pig tracheal strips.