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Adrenalin chloride solution (of Sankyo Co.), epinephrine in the bovine medullary extract prepared by Folin, Cannon and Denis and that liberated from the suprarenal bodies of dogs and cats were diluted with redistilled water, 0.85% NaCl solution, Ringer-Locke's fluid or Tyrode's fluid or the dog defibrinated blood to the strength of 1:1, 000, 000 or 1:200, 000, etc., and kept at the body temperature, that is 37 to 39.5°. The enteroinhibitory power was tested from time to time. The stability of the substances was considerably great in re-distilled water. In the normal saline solution they were also highly stable, whereas they were incomparably rapidly destroyed in the other vehicles; among them the deterioration took place most rapidly in the Tyrode's fluid. In the Locke's fluid and the dog defibrinated blood the velocity with which the evanescence progressed was nearly similar. The average velocities of the deterioration of adrenaline and epinephrine in the various vehicles are diagrammatically demonstrated in the last chart on p. 116. While in the other vehicles the deterioration went on nearly in the straight, the defibrinated blood modified the progress of destruction in the form of a curve, further destruction being arrested at the end of nearly one and a half hours of keeping warm. This is the result of the protection of the deterioration by the warmed defibrinated blood itself. The deterioration curves of adrenalin chloride, epinephrine extracted and epinephrine liberated in the dog defibrinated blood at body temperature were similar to each other. The epinephrine in the Folin's extract was somewhat more stable in the re-distilled water, normal saline, Locke and Tyrode in comparison with the adrenalin chloride of Sankyo Co.

tohoku journal of experimental medicine

On the Stability of Adrenaline Hydrochloride and Epinephrine Extracted from Bovine Suprarenal Medulla by Folin in Various Media and of Epinephrine Liberated from Suprarenal Glands of Dogs and Cats in Defibrinated Blood