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Using various albumin fortified medium, many neonatal hamster lung cells were developed into established lines. The optimal culture condition to evolve into cell lines was investigated by varying the source of albumin, concentration of serum, inoculum density, medium component and time interval. Also, the minimum culture condition necessary to sustain the serial culture was examined. The requisite in the culture condition for the development of established cell lines was discussed.

Culture Conditions Necessary to Sustain the Serial Culture of Lung Cells of Newborn Hamster in Albumin Fortified Medium