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The aim of the present work was to report the occurrence of Borrelia spp. in embryonic cell cultures from naturally infected Boophilus microplus. Seven days after the beginning of a primary culture of embryonic cells of B. microplus at 31 degrees C was noted that the cells start suffering degeneration. Under examination at phase contrast microscope, the presence of prolongated microorganisms with great mobility was detected. Microscopic slides of the culture supernatant, hemolymph and egg mass, were stained by May Grünwald-Giemsa, allowing the visualization of the spirochetes. The morphologic examination of the microorganism and its visualization in. B. microplus, suggest to be Borrelia spp.

revista brasileira de parasitologia veterinária/brazilian journal of veterinary parasitology

Ocorrência de Borrelia spp. em cultura de células embrionárias do carrapato Boophilus microplus (Acari: Ixodidae) no Estado do Mato Grosso do Sul, Brasil