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- This study investigated the effect of a supplement containing whole rice bran and crude glycerin for 21 days before mating on metabolic, productive, and reproductive responses of 28 primiparous suckling beef cows. Cows were randomly assigned to a control group (CON, n = 14), grazing on grasslands, and a supplemented group (SUP, n = 14), grazing on grasslands and supplemented daily individually with 1 kg dry matter (DM) of whole rice bran + 550 mL crude glycerin (224 g kg−1 DM of methanol) per cow. After 33 days of natural mating, cows that had not expressed estrus were subjected to a fixed-time artificial insemination protocol. Ten days after the insemination program, bulls were reintroduced for 21 days. Supplementation increased milk yield (SUP: 5.7±0.2 vs. CON: 5.0±0.2 kg d−1), milk protein content (SUP: 3.1±0.2 vs. CON: 2.8±0.2%), and body weight of cow (SUP: 379±2 vs. CON: 373±2 kg) and calf (SUP: 150±2 vs. CON: 142±2 kg). Supplementation improved the energy balance, increased plasma concentrations of cholesterol (SUP: 223.2±6.4 vs. CON: 202.1±6.4 mg dL−1) and glucose (SUP: 72.0±1.2 vs. CON: 68.6±1.2 mg dL−1), and reduced non-esterified fatty acids (SUP: 0.45±0.02 vs. CON: 0.56±0.02 mmol L−1). The percentage of cows on superficial anestrous after supplementation was greater in SUP than in CON group (57 vs. 21%, respectively); however, no difference in final pregnancy rate was found (SUP: 79 vs. CON: 64%). There was no evidence that the ingestion of crude glycerin with high content of methanol induced clinical or hepatic disorders. Supplementation of whole rice bran and crude glycerin is not toxic, and can improve the energy balance, reflecting in increase in milk yield and calf growth, with a slight effect on the reproductive activity

Supplementation with a mixture of whole rice bran and crude glycerin on metabolic responses and performance of primiparous beef cows