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The objective of this study was to evaluate the flow of nitrogenous compounds, protein degradability, rumen degradation of total carbohydrate and organic matter and microbial efficiency in heifers subjected to diets containing corn silage, sugarcane or Tifton. For this purpose, the 2,6-diaminopimelic acid (DAPA) technique was adopted and analytical procedures for amino acids by HPLC were adapted. Six rumen-fistulated Holstein-Zebu heifers with 480 kg of initial BW and at 24 months of age kept in individual tie stalls were assigned to two 3 × 3 Latin squares. Omasal digesta dry matter and microbial dry matter flows were determined using the isolated, purified and enriched lignin (LIPE®) and DAPA markers, respectively. Isolated bacteria from rumen showed on average 5.84 g/100 g microbial N, 0.25 g/100 g DAPA in dry matter and 44.61 DAPA:N ratio. The forage sources did not influence the flows of nitrogen compounds, except for total omasal flow and non-ammonia N in relation to N intake for the corn silage diet, for which there was an upward trend compared with the other diets. The degradation of the organic matter and total carbohydrates did not differ, averaging 6.1 kg/day and 5.2 kg/day, respectively. The studied forage sources do not influence the flows of nitrogen compounds, except for total omasal flow and non-ammonia N in relation N intake for the corn silage diet, for which there is an upward trend compared with the other diets. Protein degradability and microbial efficiency are similar between evaluated diets

2,6-Diaminopimelic acid (DAPA) in microbial protein quantification of heifers fed different forage sources