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Laboratory studies were carried out to develop a methodology for the mass production of Telenomus podisi Ash. and Trissolcus basalis (Woll.) on eggs of Euschistus heros (Fabr.) and to compare it with the methodology presently used in the biological control program of soybean stink bugs in Brazil [T. basalis reared on Nezara viridula (L.) eggs]. In a first experiment, egg masses randomly distributed were shown the most adequate method of exposure of E. heros to parasitism. In a second experiment, egg masses of E. heros were randomly glued on cardboard cards and exposed to parasitism by T. podisi and T. basalis. The parasitoids multiplied on E. heros eggs showed parasitism (ca. 99%) similar to that observed with T. basalis reared on N. viridula eggs (99.7%). Emergence rates were higher than 80% for T. podisi and T. basalis multiplied on E. heros eggs and 95% for T. basalis reared on N. viridula eggs. Later, similar emergence rates (> 78%) were observed on cardboard cards tied on soybean plants. No differences were detected between courtship behavior of T. podisi and T. basalis emerged from E. heros eggs compared to T. basalis emerged from N. viridula eggs. The sex ratios in the offspring of T. podisi and T. basalis emerged from E. heros eggs were similar to that exhibited by the offspring of T. basalis emerged from N. viridula eggs. The results suggest that the developed methodology is simple, efficient and can be an important tool in biological control program of soybean stink bugs.

neotropical entomology/neotropical entomology

Methodology of mass multiplication of Telenomus podisi Ash. and Trissolcus basalis (Woll.) (Hymenoptera: Scelionidae) on eggs of Euschistus heros (Fab.) (Hemiptera: Pentatomidae)