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Gamma-linolenic acid (GLA) production by Spirulina platensis under different stress-inducing conditions was studied. Submerged culture studies showed that low temperature (25ºC), strong light intensity (6 klux) and primrose oil supplement (0.8%w/v) induced 13.2 mg/g, 14.6 mg/g and 13.5 mg linolenic acid per gram dry cell weight respectively. A careful observation of fatty acid profile of the cyanobacteria shows that, oleic acid and linoleic acid, in experiments with varying growth temperature and oil supplements respectively, helped in accumulating excess &#947;-linolenic acid. In addition, cultures grown at increasing light regimes maintained the &#947;-linolenic acid to the total fatty acid ratio(GLA/TFA) constant, despite any change in &#947;-linolenic acid content of the cyanobacteria. Estudou-se a produção de ácido &#947;-linolênico por Spirulina platensis em diferentes condições de estresse. Culturas submersas indicaram que temperatura baixa (25ºC), forte intensidade de luz (6 klux) e suplementação com óleo de prímula (0,8% p/v) induziram a produção de ácido linolênico de 13,2 mg/g, 14,6 mg/g e 13,5 mg/g peso seco, respectivamente. Uma observação cuidadosa do perfil de ácidos graxos da cianobacteria indica que os ácidos oléico e linoléico, em experimentos com diferentes temperaturas de crescimento e suplementos de óleo, auxiliaram no acúmulo de excesso de ácido &#947;-linolênico. Além disso, as culturas obtidas em intensidades crescentes de luz mantiveram a relação ácido &#947;-linolênico/ácidos graxos totais constante, independentemente de qualquer mudança no conteúdo de ácido &#947;-linolênico da cianobactéria

Culture Conditions stimulating high γ-Linolenic Acid accumulation by Spirulina platensis