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In attempt to immobilize the mycelial fungi on a new hydrophobic matrix, Aspergillus terreus mycelia were immobilized into polytetrafluoroethylen (PTFE) and used for selected conversion of progesterone to 11alpha-hydroxyprogesterone. The immobilized cells exhibited higher yields than the free cells. Different parameters that can affect the cell activity were measured. The optimum pH was 6 and the optimum temperature was 30ºC. Higher transformation activities were achieved with matrix contained 40% PTFE. No considerable loss in yield was observed with storage of the cells at 4ºC for 30 days. Utilization of the immobilized cells in repeated batch processes indicated that the cells retained about 84% of their activity after reuse for eight successive cycles. Com o objetivo de imobilizar o micélio fúngico em uma nova matriz hidrofóbica, o micélio de Aspergillus terreus foi imobilizado em politetrafluoretileno (PTFE) e usado para a conversão de progesterona em 11alfa-hidroxiprogesterona. As células imobilizadas apresentaram rendimento melhor que as células livres. Diferentes parâmetros que afetavam a atividade celular foram avaliados. O pH ótimo foi 6 e a temperatura ótima foi 30ºC. Verificou-se que a atividade de transformação era mais elevada quando a matriz continha 40% de PTFT. Não houve perda significativa de atividade após o armazenamento das células a 4ºC por 30 dias. Utilizando-se as células imobilizadas em partidas repetidas, verificou-se que mantinham cerca de 84% da atividade após oito ciclos sucessivos

Production of 11alpha-hydroxyprogesterone using Aspergillus terreus immobilized on polytetrafluoroethylene