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The aim of this study was to evaluate the occurrence of thermophilic Campylobacter species in broiler flocks from 26 small properties located near the campus of Federal University of Pelotas, RS, Brazil. A total of 404 chicken fecal samples were analyzed and after isolation, identification and biotyping was performed according to Lior´s scheme. Twenty one strains (5.2%) of Campylobacter jejuni biotype II were isolated from animals from seven properties (26.9%), with a variation from 3% to 34.6% among the positive properties. We also analyzed twelve C. jejuni isolates by polymerase chain reaction (PCR), using specific primers that generate a fragment of 402 pb. Our study allowed us to observe that chickens raised in non-industrial establishments without sanitary attention can harbour C. jejuni in their intestinal tract, and by doing so, they could be a serious health risk to humans. O objetivo deste estudo foi avaliar a ocorrência de espécies termofílicas de Campylobacter em galinhas de 26 pequenas propriedades localizadas próximo ao campus da Universidade Federal de Pelotas, RS, Brasil. Um total de 404 amostras fecais de galinhas foram analisadas e após o isolamento, a identificação e a biotipificação foram realizadas de acordo com o esquema de Lior. Vinte e uma amostras (5,2%) de Campylobacter jejuni biotipo II foram isoladas de animais de sete propriedades (26,9%), com uma variação de 3,3% a 34,6% entre as propriedades positivas. Primers específicos, que geram um fragmento de 402pb, foram usados em PCR para analisar 12 isolados de C. jejuni. Concluímos que galinhas criadas em estabelecimentos não industriais e sem atenção sanitária, são portadoras de C. jejuni e, desta forma, podem ser consideradas um fator de risco para infecção humana

Campylobacter jejuni occurrence in chicken fecal samples from small properties in Pelotas, southern of Brazil