English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Three culture media were studied for the bacteriocin like substance (BLS) production from Carnobacterium piscicola L 103 in a batch and continuous culture system. The efficacy of the antagonistic substance against Listeria monocytogenes was tested on vacuum packaged salmon. BLS was produced in a 1.0 L bioreactor by batch and continuous culture using D-MRS, mod. D-MRS and APT as nutrient broths. Salmon fillets were inoculated with BLS (200 AU mL-1 and 800 AU mL-1) and 8.0 x 10¹ cfu cm-2 of L. monocytogenes and stored at 4ºC. The growth of L. monocytogenes was determined every 5 days during 15 days. After 12 h of batch culture the stationary growth phase of C. piscicola L 103 started, with a BLS activity of 800 AU mL-1 in D-MRS and mod. D-MRS broth, and of 400 AU mL-1 in APT broth. During continuous culture BLS activity increased to 6400 AU mL-1 in both types of MRS broths, while in APT the activity decreased to 50 AU mL-1, indicating a clear advantage of the first two culture media and also of the continuous culture system. BLS had a bacteriostatic effect on L. monocytogenes when inoculated on salmon, with counts of 6.0 x 10³ cfu cm-2 after 15 days. No significant differences were found between the two BLS activities used. In the control without BLS, L. monocytogenes counts increased to 1.0 x 10(6) cfu cm-2 after 15 days of storage. Três meios de cultura foram avaliados quanto a produção de substâncias semelhantes a bacteriocinas (SSB) por Carnobacterium piscicola L 103, utilizando um sistema contínuo de cultura. A eficácia da substância antagonista contra Listeria monocytogenes foi testada em salmão embalado a vácuo. As SSB foram produzidas em bioreator de 1.0 L, em cultura contínua e em cultura estacionária, utilizando D-MRS, mod. D-MRS e APT como meios de cultivo. Filés de salmão foram inoculados com SSB (200 AU mL-1 e 800 AU mL-1) e L. monocytogenes (8.0 x 10¹ ufc cm-2) e mantidos a 4ºC. O crescimento de L. monocytogenes foi verificado a cada 5 dias, durante 15 dias. C. piscicola L 103 antigiu a fase estacionária depois de 12 h de incubação em cultivo em batelada, sendo a atividade SSB de 800 AU mL-1 nos meios D-MRS e mod. D-MRS e de 400 AU mL-1 no caldo APT. Durante a cultura continua, a atividade SSB aumentou até 6400 AU mL-1 nos dois tipos de caldo MRS, ao passo que no caldo APT esta atividade diminuiu a 50 AU mL-1, indicando uma clara vantagem de uso dos dois primeiros meios de cultura e do sistema de crescimento contínuo. SSB mostrou efeito bacteriostático sobre L. monocytogenes quando inoculada em salmão, com contagens de 6,0 x 10³ ufc cm-2 após 15 dias. Não foram encontradas diferenças significantes entre as duas atividades utilizadas. No ensaio controle, sem SSB, a contagem de L. monocytogenes aumentou até 1,0 x 10(6) ufc cm-2 após 15 dias de estocagem

brazilian journal of microbiology

Bacteriocin like substance production by Carnobacterium piscicola in a continuous system with three culture broths. Study of antagonism against Listeria monocytogenes on vacuum packaged salmon