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Mutants of Aspergillus niger N402, induced by UV mutagenesis, were selected and tested for resistance or sensitivity to 5-fluorocytosine. Some mutants showed increased citric acid production, which did not correlate with the intracellular amount of protein or ammonium ion. The resistance to 5-fluorocytosine proved to be a rational approach for isolation of new mutants with improved production of citric acid. The best mutant (FR13) accumulated double the amount of citric acid produced by the parental strain. Através de mutagênese com UV, foram isolados mutantes de Aspergillus niger, verificando-se sua resistência ou sensibilidade à 5-fluorocitosina. Alguns mutantes apresentaram um aumento na produção de ácido cítrico sem um aumento correspondente da quantidade de proteína intracelular ou de ions amônio. A resistência à 5-fluorocitosina mostrou ser uma abordagem racional para o isolamento de mutantes com maior capacidade de produção de ácido cítrico. O melhor mutante selecionado, FR13, produziu o dobro da quantidade de ácido cítrico em relação à linhagem parental

brazilian journal of microbiology

&lt;![CDATA[&lt;B&gt;Selection of 5-fluorocytosine-resistant mutants from an &lt;I&gt;Aspergillus niger&lt;/I&gt; citric acid-producing strain&lt;/B&gt;]]&gt;