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The mycotoxin patulin causes gastroinstestinal distress, neurotoxic and immunotoxic effects in animals. It can be produced by several species of Penicillium, Aspergillus and Byssochlamys and it has been found in fruits, vegetables and cereals. Verruculogen is a toxin produced mainly by Penicillium and Aspergillus spp. and causes severe tremors in affected animals. Tomatoes are especially susceptible to fungi invasion and their products need to be investigated for possible mycotoxin contamination. A method for the determination of patulin and verruculogen in tomato products was developed involving an extraction with ethyl acetate, a cleanup by silica gel column and determination and confirmation by high performance liquid chromatography with diode array detector. The quantification limits of the method, defined as the minimum amount that allowed quantification and confirmation by the DAD detector, were 10 ng/g and 20 ng/g. The average recovery for patulin at five levels of addition (from 20 to 200 ng/g) was 75% and at the single level of 100 ng/g was 90 % .The average recovery for verruculogen at five levels of addition (from 50 to 300 ng/g) was 54% and at the single level of 100 ng/g was 52%. The processing of two tomato plants was followed during 1996, 1997, and 1998. Eighty-four samples of tomato pulp were analyzed for patulin and verruculogen. The toxins were not detected in any of the samples. A micotoxina patulina causa distúrbios gastrointestinais e efeitos neurotóxicos e imunotóxicos em animais. Pode ser produzida por várias espécies de Penicillium, Aspergillus e Byssochlamys e tem sido encontrada em frutas, verduras e cereais. Verruculogeno é uma toxina produzida principalmente por espécies de Penicillium e Aspergillus e causa fortes tremores em animais afetados. Tomates são especialmente susceptíveis a invasão fúngica e seus produtos precisam ser investigados com relação a possíveis contaminações por micotoxinas. Um método para determinação de patulina e verruculogeno em produtos de tomate foi desenvolvido envolvendo uma extração com acetato de etila, uma limpeza em coluna de sílica gel e determinação e confirmação por cromatografia de alta eficiência com detector de arranjo de diodos. Os limites de quantificação foram de 10 ng/g e 20 ng/g e as recuperações medias foram de 89% e 65% para patulina e verruculogeno, respectivamente. O processamento de duas fábricas de produtos de tomate foi amostrado durante 1996, 1997 e 1998. Oitenta e quatro amostras de polpa de tomate foram analisadas para patulina e verruculogeno. As toxinas não foram detectadas em nenhuma das amostras

The development of an analytical method for two mycotoxins, patulin and verruculogen, and survey of their presence in commercial tomato pulp