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In order to investigate further the adaptive response of moulds to ambient pH, we have measured by ELISA the pho-2-encoded Pi-repressible alkaline phosphatase synthesised by Neurospora crassa. We showed that the 74A and pho-2A strains of this mould secrete similar amounts of the pho-2-encoded enzyme irrespective of ambient pH, when both the preg and pgov genes are not functional, i.e., in strains nuc-2+ growing under Pi-starvation. This suggests that pho-2, which is responsive to Pi starvation via the action of genes nuc-2, preg, pgov and nuc-1, is not a gene responsive to ambient pH and that the differential glycosylation observed for the Pi-repressible alkaline phosphatase retained by the mycelium at pH 5.6 or secreted into the growth medium at pH 8.0 is the genetic response to ambient pH sensing in N. crassa. Para investigar a resposta adaptativa ao pH ambiente em fungos, foram determinados por ELISA os níveis de fosfatase alcalina Pi-repressível expressada pelo gene pho-2 de Neurospora crassa. Foi mostrado que as linhagens 74A e pho-2A deste fungo secretam quantidades semelhantes da fosfatase alcalina Pi-repressível independentemente do pH ambiente, quando ambos os genes preg e pgov não são funcionais, isto é, quando a linhagem nuc-2+ cresce em condições de limitação em fosfato inorgânico (Pi). Isto sugere que o gene pho-2, o qual é regulado pela ação hieráquica dos genes nuc-2, preg, pgov e nuc-1, é reprimido pelo fosfato inorgânico, mas não responde ao pH ambiente, e que a diferença na glicosilação observada para a fosfatase alcalina Pi-repressível (APase) retida no micélio em pH 5,6 ou APase secretada no meio de cultura em pH 8,0 é a resposta genética para o monitoramento do pH ambiente em N. crassa

The synthesis of Phosphate-repressible alkaline phosphatase do not appear to be regulated by ambient pH in the filamentous mould Neurospora crassa