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The growth of thirty-four Lentinula edodes strains submitted to different mycelial cultivation conditions (pH and temperature) was evaluated and strain variability was assessed by RAPD molecular markers. The growth at three pH values (5, 6 and 7) and four different temperatures (16, 25, 28 and 37ºC) was measured using the in vitro mycelial development rate and water retention as parameters. Mycelial cultivation was successful at all pH tested, while the ideal temperature for mycelial cultivation ranged between 25 and 28ºC. The water content was lower in strains grown at 37ºC. Among 20 OPA primers (Operon Technologies, Inc.) used for the RAPD analyses, seventeen presented good polymorphism (OPA01 to OPA05, OPA07 to OPA14, OPA17 to OPA20). The clustering based on similarity coefficients allowed the separation of strain in two groups with different geographic origins. Analisou-se o comportamento de 34 linhagens de Lentinula edodes, quando submetidas a diferentes condições de cultivo micelial (pH e temperatura), e a variabilidade das linhagens através de marcadores moleculares RAPD. A caracterização das linhagens foi realizada observando-se o comportamento de cada uma delas quando submetidas a três pHs (5, 6 e 7) e quatro temperaturas diferentes (16, 25, 28 e 37ºC), em função da taxa de desenvolvimento e da retenção de água pelo micélio in vitro. O cultivo micelial foi bem sucedido em todos os pHs utilizados. Já para o fator temperatura, foi estabelecido como faixas ideais para cultivo micelial, aquelas compreendidas entre 25 e 28ºC. O conteúdo hídrico foi menor nas linhagens cultivadas a 37ºC. O padrão de RAPD foi determinado e foram testados 20 primers OPA (Operon Technologies, Inc.), dos quais 17 forneceram boa fonte de polimorfismo (OPA01 a OPA05, OPA07 a OPA14, OPA17 a OPA20). O agrupamento das linhagens baseado nos coeficientes de similaridade permitiu a separação de dois grupos de procedências geográficas distintas

ANALYSES OF GENETIC VARIABILITY IN LENTINULA EDODES THROUGH MYCELIA RESPONSES TO DIFFERENT ABIOTIC CONDITIONS AND RAPD MOLECULAR MARKERS