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The present work had as objective to verify the genetic diversity among strains of Trypanosoma cruzi isolated in chronic chagasic patients in the Paraná state. Fifty patients with compatible clinical symptoms were selected (cardiopathy, megacolon and/or megaesophagus) and that presented positive serological reaction to T. cruzi. Six strains of the protozoan were isolated in hemoculture and identified by electrophoresis in starch thick gel with aid of 14 isoenzymes (6PGD; G6PD; ME¹; ME²; ICD; PGM; GPI; GOT¹; GOT²; NP¹; NP²; DIA; MPI and FH). The statistical analysis were accomplished by the softwares NTSYs and UPGMA. Eighty-four electromorphs were individualized and four isoenzymes (ICD, GPI, 6PGD, e PGM) showed compatibility for heterozygosis. The phenetic analysis evidenced the hypothesis of constant evolution (genetic distances = 0.0536 to 0.1429, Hardy-Weinberg = 1.0000 to 0.0769 and differentiation for exact tests = 1.0000 to 0.0658). A great intra-specific genetic diversity in T. cruzi was verified in the isolates obtained in humans and it indicates that the clonet II is associated to the domestic cycle of transmission. O presente trabalho teve como objetivo verificar a diversidade genética entre cepas de Trypanosoma cruzi circulantes em pacientes chagásicos crônicos no estado do Paraná. Foram selecionados 50 pacientes com clínica compatível (cardiopatia, megacolo e/ou megaesôfago) e que apresentaram reação sorológica positiva para T. cruzi. Foram isoladas seis cepas do protozoário em hemocultura e identificadas por eletroforese em gel espesso de amido com auxílio de 14 isoenzimas (6PGD; G6PD; ME¹; ME²; ICD; PGM; GPI; GOT¹; GOT²; NP¹; NP²; DIA; MPI e FH). A análise estatística foi realizada pelos programas NTSYs e TFPGA. Oitenta e quatro eletromorfos foram individualizados sendo que quatro isoenzimas (ICD, GPI, 6PGD, e PGM) apresentaram compatibilidade para heterozigose. A análise fenética evidenciou a hipótese de uma evolução constante (distâncias genéticas = 0,0536 a 0,1429, Hardy-Weinberg = 1,0000 a 0,0769 e diferenciação por exatos testes = 1,0000 a 0,0658). Uma grande diversidade genética intra-específica em Trypanosoma cruzi foi verificada nos isolados obtidos em humanos. È indispensável conhecer se há ou não inter-relação entre o ciclo doméstico e ciclo silvestre deste protozoários

Trypanosoma cruzi Chagas, 1909: genetic variability of isolates from chronic chagasic patients in the Paraná state, Brazil