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The extraction of alpha-amylase in the solid state fermentation of wheat bran by Bacillus circulans GRS313 was optimized. Among various solvents tested, maximum extraction was achieved when 2.5% (v/v) glycerol was added. The optimum conditions for extraction were 2.5 hrs soaking time at 30° C under recirculation and agitated condition, which yielded 590 U/g bran of enzyme activity. Whereas under stationary soaking condition the activity of the enzyme was 439.58U/g bran. With repeated wash under the above optimum conditions showed that 600 U/g and 184.2 U/g of enzyme extracted in the 1st and 2nd washes respectively and only 36.84 U/g was recovered in the 3rd wash Otimizou-se a extração de alfa- amilase na fermentação no estado sólido do farelo de trigo Bacillus circulans GRS313 . Entre vários solventes testados, conseguiu-se a melhor extração com solução 2,5% (v/v) de glicerol. As condições ótimas de extração foram de 2,5 horas de tempo de contato a 30° C sob condições de agitação e recirculação, chegando a uma atividade enzimática de 590 U/g farelo, enquanto chegou-se a uma atividade enzimática de 439.58U/g farelo sob condições de contato estacionárias. Com lavagens repetidas nas condições ótimas descritas acima, chegou-se à uma atividade enzimática de 600 U/g farelo e 184,2 U/g de enzima extraída nas 1 ª e 2ª lavagens, respectivamente, e apenas 36,84 U/g recuperada na 3 ª lavagem

Optimization of extraction parameters for recovery of alpha-amylase from the fermented bran of Bacillus circulans GRS313