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A total number of 300 females and 30 males of two experimental broiler lines, TT (male) and PP (female), with 39 and 36 weeks of age, respectively. Experiment 1 (TT) evaluated the effect of storage time (ST) on hatchability and embryo mortality (EM). Eggs were stored for 2 (ST1), 4 (ST2), 6 (ST3), 8 (ST4), 10 (ST5), 12 (ST6), and 24 (ST7) days and EM was assessed in the periods of 0 to 6 (EM1), 7 a 13 (EM2), 14 to 17 (EM3), 18 to 21 (EM4), and 0 to 21 (TEM) days. Experiment 2 (PP) evaluated the effect of egg weight (EW) on embryo development. Eggs were individually collected and weighed. Embryos were collected and weighed on days 9 (EW9), 11 (EW11), 13 (EW13), EW15, 17 (EW17) days of incubation and at hatching (EW21). ST linearly influenced hatchability and EM, with an estimated 1.17% reduction and a 1.15% increase, respectively for each 1.0 day of storage. Hatchability was reduced in 21% betweeb ST2 and ST7, resulting from a 462% increase in EM. The correlation between egg weight and embryo weight (EW) was significant after EW3 (0.25), reaching 0.72 for EW21, which corresponded to 70.92% of egg weight. The estimated increase in embryo weight for each 1.0g increase in egg weight was 0.71g for EW21

Effect of storage period and egg weight on embryo development and incubation results