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Two experiments were carried out to evaluate the effects of particles sizes on contents of neutral detergent fiber (NDF) by using nylon bags F57 (Ankom®) and non-woven textile (NWT 100 g/m²). In the first experiment, to check contents of NDF obtained with each one of the textilles, quantitative filter paper (purified cellulose) was used as analytical standard. The material was processed in a 1 or 2 mm and put in the bags in a ratio of 20 mg of dry matter/cm² of surface. The analyses were performed in a fiber analyser (Ankom220) and using a heat-stable a-amylase. No effects of particles sizes were found. In the second experiment, it was used samples of forage (signal grass hay, sugar cane, coastcross hay, corn straw, corn silage, and elephant grass at 50 and 250 days of growing after cutting), concentrate feeds (sorghum grain, gluten meal, citrus pulp, cottonseed meal, soybean meal, wheat bran, corn grain, whole soybean, and soybean hulls), and feces of cattle of animals fed diets containing 15 or 50% of concentrate. The samples were prepared, put in bags, and analyzed as described in the previous experiment. In both experiments, the smallest contents of NDF were found by using nylon bags, indicating loss of particles through the bag porosity. It is suggested grind of samples by using 1-mm screen sieve, which provides efficient extraction of cell content by the action of neutral detergent and a greater specific surface by the action of the heat-stable a-amylase enzyme. The use of particles ground at 2-mm overestimates NDF contents. Foram realizados dois experimentos objetivando-se avaliar a influência do tamanho das partículas sobre os teores de fibra em detergente neutro (FDN) utilizando-se sacos de náilon, F57 (Ankom®) e tecido-não-tecido (TNT 100 g/m²). No primeiro experimento, com o intuito de aferir os teores de FDN obtidos com cada um dos tecidos, utilizou-se papel-filtro quantitativo (celulose purificada) como padrão analítico. O material foi processado em 1 e 2 mm e acondicionado nos sacos seguindo-se a relação de 20 mg de matéria seca/cm² de superfície. Utilizou-se aparelho analisador de fibras (Ankom220), empregando-se a-amilase termoestável. Não foram verificados efeitos do tamanho de partículas. Contudo, perdas de partículas significativas foram verificadas com o uso do náilon. No segundo experimento foram utilizadas amostras de forragem (feno de capim-braquiária, cana-de-açúcar, feno de coastcross, palha de milho, silagem de milho e capim-elefante cortado aos 50 dias e 250 dias de rebrotação), alimentos concentrados (sorgo grão, glúten de milho, polpa cítrica, farelo de algodão, farelo de soja, farelo de trigo, milho grão, soja grão e casca de soja) e fezes bovinas oriundas de animais alimentados com dietas contendo 15 e 50% de concentrado. As amostras foram preparadas, acondicionadas e analisadas como descrito no experimento anterior. Os menores teores de FDN foram encontrados com o uso de sacos de náilon, o que indica perda de partículas pela porosidade do saco. Recomenda-se a moagem de amostras em peneiras de porosidade 1 mm, o que propicia a extração eficiente do conteúdo celular pela ação do detergente neutro e maior superfície específica para ação da a-amilase termoestável. O uso de partículas processadas a 2 mm conduz à superestimação dos teores de FDN

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Evaluation of neutral detergent fiber contents in forages, concentrates and cattle feces ground at different particle sizes and using bags made from different textiles