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Objetivou-se avaliar a influência do peso vivo de novilhas sobre o consumo e a digestibilidade da MS, MO, EE, PB, fibra em detergente neutro corrigida para cinzas e proteína (FDNcp) e carboidratos não-fibrosos corrigidos (CNFcp), os consumos de NDT e de proteína degradada no rúmen (PDR), a produção de proteína microbiana e a excreção de compostos nitrogenados na urina e comparar as metodologias de coleta spot e de coleta total de urina. Foram utilizadas 22 novilhas com cinco diferentes pesos (523, 453, 315, 235 e 118 kg), distribuídas em delineamento inteiramente casualizado, com cinco tratamentos (P523, P453, P315, P235 e P118). A alimentaço foi constituída de silagem de milho e concentrado, fornecido na proporção de 1,5 kg/dia (tratamentos P523, P453, P315 e P235) ou 2 kg/dia (tratamento P118). Os consumos dos nutrientes em kg/dia e em %PV aumentaram e diminuíram linearmente, respectivamente, com o aumento do peso dos animais. As digestibilidades da MS, MO e o teor de NDT não diferiram entre os tratamentos, enquanto as de PB e FDNcp foram distintas entre os tratamentos, sendo maior e menor, respectivamente, no tratamento P118. A excreção de uréia na urina (em mg/kgPV) foi maior no tratamento P118, variando com o peso vivo e com o consumo de PB e PDR. A produção microbiana, estimada pela excreção urinária de derivados de purinas, não diferiu entre as metodologias de coleta spot e coleta total de urina, sendo menor no tratamento P118. A coleta de urina spot pode ser utilizada para estimativa da excreção de compostos nitrogenados na urina, independentemente do peso vivo. A quantidade de nutrientes ingeridos diariamente aumenta com o peso vivo, entretanto, o consumo desses nutrientes, expresso em %PV, diminui à medida que o peso vivo aumenta. The objective of this trial was to evaluate the effect of body weight (BW) of heifers on intake, apparent digestibility of nutrients, microbial protein synthesis, ruminal metabolism, and urinary excretion of nitrogenous compounds. The spot urine sampling and the total collection of urine techniques were used to estimate microbial N flow. Twenty-two heifers with different body weights (523, 453, 315, 235 and 118 kg) were used in a completely randomized design and assigned to the following treatments: BW523, BW453, BW315, BW235 or BW118. Animals were fed corn silage ad libitum plus 1.5 kg/day of concentrate on treatments BW523, BW453, BW315 and BW235 and 2 kg/day on BW118 treatment. Intake of nutrients increased linearly from treatment BW118 to BW523. However, the opposite was observed when DMI was expressed as percentage of BW. Digestibilities of DM and OM and dietary TDN did not differ among treatments but digestibilities of CP and NDF corrected for ash and protein (NDFap) were greatest and lowest on BW118 treatment, respectively. The urinary excretion of urea (mg/kgBW) was higher on BW118 heifers and varied with BW and dietary contents of CP and RDP. Microbial protein synthesis was not affected by sampling methodology and was lowest on treatment BW118. Therefore, spot urine sampling can be used to estimate urinary excretion of nitrogenous compounds and microbial protein synthesis

Consumo, digestibilidade e excreção de uréia e derivados de purinas em novilhas de diferentes pesos