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To estimate nutrient and energy digestion with animal from in vitro variables it is necessary to control factors that may interfere on digestion such as: source and concentration of digestor substance, as well as the origin and processing of test ingredients. In this study there were performed five laboratory assays, two with poultry and finally regression equations were established based on variables generated with twelve vegetable ingredients. The results showed that there is greater solubility of proteins with NaOH than with KOH. On average, both alkali produced better discrimination of soluble protein with concentration of 0.02% (range of 68.61%) in relation to a concentration of 0.2% (range of 37.97%). Considering interaction between alkali and concentration, the best discrimination on protein solubility was reached with NaOH at 0.02%. The methods of protein or fiber solubilization together with the chemical analysis showed significant results variation among ingredients and when they were used to estimate in vivo variables they produced good estimates. It was concluded that reliable digestion estimates for energy or amino acids from vegetable feed ingredients could be obtained from in vitro variables, when considering solubilities of protein and fiber with chemical analysis. Para estimar a digestão de nutrientes e de energia dos animais a partir de variáveis in vitro, é necessário controlar os fatores que interferem na digestão - origem e concentração da substância digestora - e ter conhecimento da fonte e do processo que envolve o ingrediente em teste. Neste trabalho, foram conduzidos cinco ensaios laboratoriais, dois com animais e, por fim, estabelecidas equações de regressão a partir das variáveis geradas com a utilização de 12 ingredientes vegetais. Os resultados mostraram que há maior solubilidade das proteínas com o NaOH que com o KOH e que, na média de ambos os álcalis, a concentração de 0,02% permite maior discriminação na solubilidade das proteínas (diferença de 68,61%) em relação a 0,2% (37,97%). Considerando a interação entre álcalis e concentrações, a melhor discriminação foi com NaOH a 0,02%. Os métodos de solubilização da proteína ou fibra, juntos com as análises bromatológicas, mostraram variações significativas e, quando usados para relacionar as variáveis in vivo, produziram bons resultados estimadores. Cncluiu-se que podem ser obtidas estimativas confiáveis da digestão animal de ingredientes vegetais com a determinação de variáveis in vitro, desde que considerados fatores relacionados à solubilidade da proteína e fibra e variáveis químico-bromatológicas

Metabolizable energy and amino acids relationships with the soluble fractions of protein and fiber of vegetable feed ingredients