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The study was carried out in order to evaluate the effect of temperature and in vitor stigma receptivity on regeneration of orange cultivar (Valência, Pêra and Natal) pollen. Two experiments were carried out, in the first the ideal temperature of germination was assessed. Pollen was obtained from flowers at the balloon stage and inoculated in culture medium with 10 g L-1 agar, 200 mg L-1 boric acid, 100 g L-1 sucrose, 800 mg L-1 calcium nitrate, pH adjusted to 6,5 and incubated in a BOD chamber at temperatures of 23, 24, 25, 26 and 27ºC during a 24-hour photoperiod. After 12 hours of incubation, the best temperature for pollen grain germination was 25ºC for all varieties. In a second experiment, in order to test the receptivity of the stigma, flowers were collected at different flowering stages: small bud, balloon and open flower. The stigmas were by immersion exposed to hydrogen peroxide (perioxidase reaction), 3% for 3 minutes. Through the technique of Zeisler (1938), better results were detected at the balloon stage with 80 to 100% receptivity, while for the open flower the receptivity presented maximum indexes. Objetivou-se determinar a melhor temperatura para a germinação de grãos de pólen e avaliar a receptividade do estigma das cultivares cítricas Valência, Pêra e Natal. Para avaliar a temperatura ideal de germinação, grãos de pólen foram obtidos de flores em estágio "balão" e inoculados em meio de cultura constituído por 10 g L-1 de ágar, 200 mg L-1 de ácido bórico, 100 g L-1 de sacarose, 800 mg L-1 de nitrato de cálcio, pH ajustado para 6,5 e incubado nas temperaturas de 23, 24, 25, 26 e 27ºC em estufa tipo D.B.O. e fotoperíodo constante de 24 horas. Após 12 horas de incubação, observou-se que a melhor temperatura de germinação de grãos de pólen é de 25ºC para todas as variedades. Para avaliar a receptividade do estigma, foram coletadas flores em diferentes estádios de florescimento: botão pequeno, balão e flor aberta. Os estigmas foram retirados e imersos em água oxigenada a 3% por 3 minutos. A liberação de bolhas de ar indica que o estigma está receptivo. Melhores resultados foram obtidos com a técnica de Zeisler (1938), no estágio de balão com 80 a 100% de receptividade, ao passo que a receptividade para a flor aberta apresentou os índices máximos

Receptiveness of the stigma and in vitro germination of orange pollen, submitted to different temperatures