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Escherichia coli faz parte da microbiota anaeróbica facultativa normal, sendo também considerada um dos maiores patógenos entéricos predominantes no cólon dos animais e homem. Neste trabalho, realizaram-se ensaios "in vitro" para avaliar o grau de atividade antagonista de cinco cepas de Lactobacillus acidophilus, com capacidade probiótica sobre Escherichia coli BIA 26 (STEC) isolada de queijo "Minas Frescal". Para tanto, foi utilizado o teste de inibição através do método de dupla camada em triplicata para avaliar zonas de inibição de crescimento. Todas as cepas de Lactobacillus mostraram-se capazes de inibir a E. coli, com zonas de inibição variando de 12 a 15mm de diâmetro, sendo que a maioria apresentou 14mm de diâmetro, consideradas como fortes halos de inibição. Escherichia coli is part of the normal facultative anaerobic microflora and one of the major enteric pathogens in the colon of animals and humans. In vitro trials were conducted to evaluate the level of antagonistic activity of five strains of Lactobacillus acidophilus with probiotic effect on Escherichia coli BIA 26 (STEC), which was isolated from 'Minas frescal' cheese. Inhibition assays were carried out using the double layer method in triplicates to evaluate growth inhibition zones. All L. acidophilus strains were capable to inhibit E. coli, with inhibition zones ranging from 12 to 15mm, but mostly around 14mm in diameter, and considered strong inhibition halos

Inibição do crescimento de Escherichia coli isolada de Queijo "Minas Frescal" por Lactobacillus acidophilus