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Existem variações entre genótipos de trigo quanto à regeneração de plantas in vitro. Em trabalho precursor com o genótipo Maringá obteve-se 100% de regeneração de plantas. Este trabalho visou investigar a capacidade de regeneração de plantas do Maringá e duas linhas isogênicas com um e dois genes de estatura reduzida (Rht1 e Rht2). Foram utilizados embriões imaturos de Maringá, das linhas isogênicas e das F2's dos cruzamentos entre estes genótipos, que foram colocados em meio de cultura MS modificado (MILACH et al., 1991) com sacarose, ágar e doses decrescentes de 2,4D conforme a etapa de cultivo (2mg/l; 0,5mg/l; 0,1mg/l e sem 2,4D). Também foi avaliada a possibilidade de supressão de um subcultivo, quando os calos não passariam pelo meio com 0,1mg/l de 2,4D. As análises estatísticas demonstraram que os genótipos diferiram quanto à regeneração de plantas, evidenciando a influência do gene Rht, que reduziu o número de plantas regeneradas por calo. A retirada do meio com 0,1mg/l de 2,4D não alterou a regeneração, podendo-se eliminar esta etapa de cultivo. Os genótipos responderam da mesma forma nas diferentes sequências de subcultivo, não havendo interação entre estes dois caracteres. Different genotypes show different responses for in vitro plant regeneration. In previous work with lhe Maringá genotype a result of 100% of in vitro plant regeneration was obtained. The purpose of this study was to investigate the capacity of plant regeneration of Maringá genotype and two of it's isogenic lines with one and two shorts heigth genes (Rht1 and Rht2). Imature embryos from: (a) Maringá, (b) the isogenic lines and (c) the crosses between these genotypes were placed on MS medium (modified by MILACH et al., 1991) with saccharose, agar and decreasing doses of 2,4D (2mg/l; 0.5mg/l; 0.1mg/l and 0.0mg/l) according to the stage of culture. The possibility of suppressing one of the stages of culture, (which uses 0.1mg/1 of 2.4D) was also evaluated. The statistic analisys demonstrated that the different genotypes gave different responses, therefore this indicates that the Rht genes had influence on plant regeneration rates. The suppression of the stage of culture which uses 0.1mg/l of 2,4D did not have any influence on regeneration, showing that this stage can be eliminated. Different genotypes had the same responses on the different culture medium sequences. The intercection between genotype x medium was not significam

Regeneração de plantas de trigo (Triticum aestivum L.) de linhas isogênicas do genótipo Maringá com genes de porte baixo (Rht1 e Rht2)