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In the present study, periapical radiographs were analyzed to investigate the number of canals of the buccal root of maxillary 1st premolars with longitudinal sulcus, and compare the number of apical foramina observed on the radiographs to that revealed by scanning electron microscopy (SEM). Thirty-nine human maxillary 1st premolars had their crowns and palatal roots removed and the root canals (C) were explored with endodontic files until the tip of the instrument was visualized exiting the apical foramen/foramina (AF). Radiographs were taken with files inside the root canals and the specimens were classified as follows: 1C/1AF; 2C/2AF; 1C/2AF; 2C/1AF. Apices were examined under SEM and the number of apical foramina (considering those measuring &gt;80 microm in diameter) was recorded. Only 17.1% (n=6) of the specimens had 1C/1AF; 77.2% (n=27) had 2AF in which 42.9% (n=15) had 2C and 34.3% (n=12) had 1C; 5.7% (n=2) had 2C/1AF. SEM examinations showed that 46.1% (n=18) of the specimens had 2AF; 23.1% (n=9) had 1AF; 23.1% (n=9) had 3AF; 7.7% (n=3) had 4AF. Radiographic and SEM findings for the number of AF coincided in 62.8% of the cases. A buccal longitudinal sulcus in the maxillary 1st premolar is usually accompanied by total bifurcation (42.9%) or apical bifurcation (34.3%) of the root canal. SEM identified a statistically higher number of apical foramina than radiographs (p&lt;0.01).

Root canals and apical foramina of the buccal root of maxillary first premolars with longitudinal sulcus