English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Um metodo rapido e simples de LC-UV (cromatografia liquida com deteccao ultravioleta) foi desenvolvido e validado para quantificacao do 5-acridina-9-ilmetileno-3-(4-fluor-benzil)tiazolidina-2,4-diona (LPSF/AC-23) em plasma de ratos utilizando extracao por precipitacao proteica com acetonitrila, e uma tiazolidinadiona (3-(2-bromo-benzil)-5-(5-bromo-2-metoxibenzilideno)-tiazolidina-2,4-diona) como padrao interno. A separacao e quantificacao do LPSF/AC-23 foi realizada utilizando uma fase movel composta por uma mistura de acetonitrila/metanol/tampao fosfato (55:30:15) eluida de forma isocratica atraves de uma coluna analitica C 18 seguida de deteccao UV a 249 nm. A curva de calibracao foi linear na faixa de 100-10.000 ng mL –1 . A precisao intra e interdia apresentou valores de desvio-padrao relativo preconizados pela Agencia Nacional de Vigilância Sanitaria (Anvisa) e a exatidao, expressa pelo erro relativo, variou de –3,49 a 7,67%. A recuperacao foi de 92,25% para o analito e 89,67% para o padrao interno e nenhum interferente endogeno foi observado. Desta forma, o metodo proposto pode ser aplicado na determinacao quantitativa do LPSF/AC-23 em plasma de ratos em estudos farmacocineticos e de biodisponibilidade. A quick and simple LC-UV (liquid chromatography with ultraviolet detection) method was developed and validated for quantification of 2,4-thiazolidinedione,5-(9-acridinylmethylene)3-[(4-fluorophenyl)methyl] (LPSF/AC-23) in rat plasma using protein precipitation extraction with acetonitrile, and a thiazolidinedione 2,4-thiazolidinedione,5-[(5-bromo-2-methoxyphenyl) methylene]-3-[(2-bromophenyl)methyl] as an internal standard. The separation and quantification of LPSF/AC-23 were performed using a mobile phase consisting of a mixture of acetonitrile/methanol/phosphate buffer (55:30:15) eluted in an isocratic manner through a C18 analytical column followed by UV detection at 249 nm. The calibration curve was linear in the range of 100-10,000 ng mL –1 . The intra- and inter-day precisions expressed by relative standard deviation values were recommended by the Brazilian Health Surveillance Agency (Anvisa), and accuracy expressed by relative error ranged from −3.49 to 7.67%. The recovery was 92.25% for the analyte and 89.67% for the internal standard and no endogenous interference was observed. Thus, the proposed method can be applied to quantitative determination of LPSF/AC-23 in plasma of rats, in pharmacokinetic and bioavailability studies.

Development and validation of an LC-UV method for quantification of a new thiazolidinedione (LPSF/AC-23) with antitumor activity in rat plasma