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In the Northeast of Brazil the stingless bee Trigona spinipes Fabricius injures the tree bark of cultivated Eucalyptus citriodora specimens in order to make them exudate. The chemical investigation of the ethanol extract of an entire nest of T. spinipes allowed the isolation of the cycloartane triterpene magniferolic acid and 3b-hydroxy-24-methylenecicloartan-26-oic acid, besides the flavonoids 3-methyl quercetin, sakuranetin, kaempferol 7-methyl ether, tricetin and aromadendrin 7-methyl ether as the main compounds. The isolation of sakuranetin, kaempferol 7-methyl ether, and aromadendrin 7-methyl ether from both Trigonas spinipes'nest and the exudate from Eucalyptus, may suggest this species as a botanical origin of the nest constituents of these stingless bee in the Northeast of Brazil. The structural characterization of the isolated compounds was accomplished by spectrometric means and comparison with the literature data.

Flavonoids and triterpenes from the nest of the stingless bee Trigona spinipes