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Anthropogenic influences on the characteristics of aquatic dissolved organic matter (DOM) were evaluated in this work using synchronous fluorescence (SyF) scan spectroscopy. Samples from the Iraf and the Iguacu Rivers were collected during the summer and winter at points located upstream and downstream from a heavily urbanized area in Brazil, respectively. DOM from all samples was titrated with Cu2+ and monitored by SyF quenching. A 1:1 non linear model was used for data treatment in, at least, five distinct regions of the spectra. Results revealed that during the rainy summer, DOM from both rivers exhibited simple organic structures such as aromatic amino acids or conjugated aliphatics as the major constituents, probably arising from non-point sources. However, during the dry winter it became clear that raw sewage discharges arising from the urbanized area contributed to an increase of fluorescent organic matter that is unable to complex copper

Assessment of anthropogenic influences on copper complexation by aquatic dissolved organic matter using synchronous fluorescence