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Self-immobilized and radiation-immobilized stationary phases were prepared with poly(methyloctylsiloxane) on eight silica supports having different particle sizes, particle shapes and pore sizes. Columns prepared by the two immobilization procedures had similar efficiencies, resolutions and separation factors but columns with self-immobilized stationary phases had lower percent loadings and, thus, lower retention factors. Results show structural differences between the phases.Dois tipos de fase estacionária, auto-imobilizada e imobilizada por radiação, foram preparadas com poli(metiloctilsiloxano) sobre oito suportes de sílica com tamanho e forma de partículas, e tamanho de poros diferentes. As colunas recheadas com as fases estacionárias preparadas através dos dois procedimentos de imobilização apresentaram eficiências, resoluções e fatores de separação similares, mas as colunas recheadas com a fase estacionária auto-imobilizada tiveram percentagens de carga menores e, portanto, fatores de retenção menores. Os resultados evidenciam diferenças estruturais entre as fases

Chromatographic comparison of self-immobilized and radiation-immobilized poly(methyloctylsiloxane) stationary phases on various silicas