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Two fluorescence bands were detected using synchronous-scan fluorescence on samples of terrestrially derived humic substances (lex/lem 380/398, 440/458 nm) without the addition of copper. A third band (lex/lem 502/520 nm) was detected after severe quenching of the second band (lex/lem 440/458 nm) caused by additions of copper. A more energetic band (lex/lem 333/351 nm) is present in marine DOM, but absent in the terrestrial material. The effects of copper on both the intensity and shape of the synchronous-scan fluorescence spectra and this technique s potential use as a tracer are discussed.A fluorescência de substâncias húmicas de origem terrestre foi investigada através da técnica de fluorescência sincronizada, e as amostras mostraram duas bandas principais (lex/lem 380/398, 440/458 nm) quando analisadas sem adição do metal paramagnético cobre. Uma terceira banda (lex/lem 502/520 nm) foi detectada após severa extinção de fluorescência da segunda banda causada pela adição de cobre. Uma outra banda, mais energética (lex/lem 333/351 nm) foi detectada em amostras marinhas, porém ausente do material de origem terrestre. Os efeitos de cobre sobre a intensidade e forma do espectro de fluorescência sincronizado e a utilização desta técnica como marcadores são discutidas

Synchronous-scan fluorescence and the complexation of copper (II) ions by humic substances