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The present study was undertaken to evaluate the adaptogenic activity of ethanol (EtHI), ethyl acetate (EAHI) fractions of Habenaria intermedia D. Don, Orchidaceae (HI), tubers using immobilization induced acute stress (AS), chronic stress (CS) and swimming induced stress in experimental animals. The tested doses of EtHI (100 and 200 mg/kg, p.o.) and higher dose of EAHI (200 mg/kg, p.o.) normalized altered serum biochemical parameters and the severity of ulcers in both AS and CS. EAHI and EtHI restored the hyperthrophy of adrenal gland and atrophy of spleen and thymus gland in AS and CS. Greater swimming time was noted in the mice pretreated with EtHI and EAHI. Levels of adrenal ascorbic acid and cortisol were restored significantly. EAHI exhibited prominent scavenging effect of DPPH, hydroxyl radical and lipid peroxidation in vitro. Phytochemical studies resulted in the isolation of scopoletin and gallic acid as marker compounds. Our results proved the traditional claim of HI as anti-stress/adaptogen in Ayurvdea

Protective effect of Habenaria intermedia tubers against acute and chronic physical and psychological stress paradigms in rats