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The hypoosmotic test (HO) was used to evaluate plasmatic membrane integrity of equine chilled semen and to estimate the correlation between the results of the HO test and those from applied routine exams of semen. The semen of seven stallions was preserved at 5ºC in three different extenders. Each ejaculate was diluted in three extenders, Kenney (K), Baken with 3% of egg yolk (B3) and Baken with 10% of egg yolk (B10), and chilled at 5ºC. The total motility (MT), progressive motility (MP), spermatic vigor (V), normal spermatic morphology (NOR) and HO test were used to evaluate semen immediately after collection and at 24 hour-intervals. Spermatic longevity was defined as the time taken to the progressive motility to decline under 10%. Semen diluted in B3 had the best longevity. The results of the HO test suggest that extenders made with egg yolk preserved the membrane plasmatic. Correlation (P<0.05) between HO test and spermatic motility (MT=0.57; MP=0.59), and low correlation between HO and NOR (0.16) were observed. Utilizou-se o teste hiposmótico (HO) para estudar a capacidade de preservação da membrana plasmática do sêmen eqüino resfriado em diferentes meios. Estimou-se a correlação entre os resultados do teste HO e os exames de rotina aplicados ao sêmen, usando-se sêmen de sete garanhões. Cada ejaculado foi diluído em três meios, Kenney (K), Baken com 3% de gema (B3) e Baken com 10% de gema de ovo (B10), e resfriado a 5ºC. Avaliaram-se a motilidade total (MT), a motilidade progressiva (MP), o vigor espermático (V), a porcentagem de espermatozóides morfologicamente normais (NOR) e os resultados do teste HO no sêmen fresco e a cada 24 horas pós-resfriamento. A longevidade espermática foi considerada como o tempo de manutenção da motilidade espermática progressiva superior a 10% do sêmen diluído e resfriado. Maior longevidade espermática foi obtida nas amostras diluídas em meio B3. Os resultados do teste HO sugerem que os diluidores à base de gema de ovo preservaram melhor a membrana plasmática. Foram obtidos valores de correlação (P<0,05) entre o teste HO e a motilidade espermática (MT=0,57; MP=0,59), e correlação baixa entre HO e NOR (0,16)

Hypoosmotic test to predict viability of equine chilled semen in different extenders