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O presente trabalho foi realizado no laboratório e estufas plásticas da Universidade de Passo Fundo, com o objetivo de caracterizar a morfologia em folhas de morangueiro cultivar Vila Nova, durante o enraizamento in vitro, cultivado em quatro diferentes concentrações de sacarose (15; 30; 45 e 60 g.L-1 ) e, durante a aclimatização ex vitro e in vivo. Os resultados mostraram que concentrações de 30 a 45 g.L-1 otimizaram o desenvolvimento in vitro, no entanto as folhas apresentaram a mesma morfologia nas quatro concentrações de sacarose. As folhas das plantas in vitro apresentaram diferenciação no mesofilo, quando comparadas com as folhas de plantas in vivo, apresentando redução do tecido paliçádico e lacunoso assim como redução da camada de cera da cutícula. Após oito semanas de aclimatização ex vitro, as folhas das plantas apresentaram similar morfologia das folhas das plantas in vitro, porém com algumas características de plantas in vivo. This work was carried out at the laboratory and greenhouse of the Universidade de Passo Fundo (Brazil), to evaluate the strawberry leaves morphology of cv. Vila Nova during rooting in vitro (growing on 15; 30, 45, and 60 g L-1 of sucrose), and to evaluate the strawberry leaves morphology during the acclimatization ex vitro and in vivo. A better in vitro plants development was found by using 30 and 45 g L-1 of sucrose, while no differences were found for the leaf morphology in different sucrose concentrations. Leaves from plants grown in vitro showed mesophyl tissues differentiation when compared to the in vivo plants. The latter showed a decrease of the palisade and spongy tissues, as well as, a decrease of the epicuticular wax layer. After eight weeks of acclimatization ex vitro, the leaves showed a similar morphology to the in vitro plant leaves, however, some characteristics of in vivo plants were still present

Análises anatômicas e da biomassa em plantas de morangueiro cultivadas in vitro e ex vitro