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We carried out a mammalian survey in the neighborhoods of the Serra da Bodoquena National Park, Mato Grosso do Sul state, a region poorly known in southwestern Brazil. During the months of April, May and July 2002 we used wire live trap, direct observation, indirect evidence (e.g. tracks), carcasses, and interviews with local residents to record mammalian species. Fifty six mammal species were recorded, including threatened species (14%). These records were discussed regarding species abundance, distribution, range extension, habitat, and conservation. The geographic distribution and ecology of the poorly known marsupials Thylamys macrurus and Micoureus constantiae in Brazil are emphasized. Efetuamos um levantamento de espécies de mamíferos no entorno do Parque Nacional da Serra da Bodoquena, Estado de Mato Grosso do Sul, uma região pouco conhecida no sudoeste do Brasil. Durante os meses de abril, maio e julho de 2002 efetuamos captura com armadilhas, observação direta, busca por evidências indiretas (e.g. pegadas), carcaças, e entrevistas com moradores locais para registrar espécies de mamíferos. Cinqüenta e seis espécies foram registradas, incluindo ameaçadas de extinção (14%). Discutimos abundância, distribuição geográfica, preferência de habitat e conservação das espécies registradas. A distribuição geográfica e ecologia dos marsupiais Thylamys macrurus e Micoureus constantiae no Brasil são enfatizadas

Mammals of the Bodoquena Mountains, southwestern Brazil: an ecological and conservation analysis