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Changes in the polyamine content were analyzed in different embryo developmental stages and tissues during seed development in the conifer Araucaria angustifolia (Bert.) O. Ktze. Free polyamine contents varied according to the tissue and stage of embryo development, the highest levels occurring in the embryonic axis at the early stages, when putrescine and spermidine were most abundant. The levels of spermidine were higher from the stage where cotyledons arise, whereas putrescine decreases. The putrescine/spermine+spermidine ratio was higher during the initial phases of seed development, corresponding to cell multiplication and elongation, with a decrease in the final stages, corresponding to stabilization of the dry matter content. As variações nos conteúdos de poliaminas foram analisadas em diferentes estágios e tecidos durante o desenvolvimento da semente de Araucaria angustifolia (Bert.) O. Ktze. O conteúdo de poliaminas livres variou de acordo com o tecido e o estágio de desenvolvimento, com os maiores níveis observados no eixo embrionário nos estágios iniciais, sendo a putrescina e espermidina as mais abundantes. A partir do estágio de aparecimento dos cotilédones, o nível de espermidina aumentou, enquanto o nível de putrescina decresceu. A relação putrescina/spermina+espermidina foi mais elevada durante as fases iniciais do desenvolvimento da semente, correspondendo à multiplicação e alongamento, com um decréscimo nos estágios finais, quando ocorre a estabilização do conteúdo da matéria seca

Changes in polyamines content associated with zygotic embryogenesis in the Brazilian pine, Araucaria angustifolia (Bert.) O. Ktze.