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O experimento teve como objetivo caracterizar biológica e molecularmente os isolados de CTV, obtidos de árvores de laranja Pêra em pomares das regiões Norte e Noroeste do Paraná, visando selecionar isolados fracos do Citrus tristeza virus com capacidade protetiva para pré-imunização. Os resultados apontaram sintomas fracos a moderados de caneluras e revelaram similaridade genética da maioria dos isolados analisados e o isolado fraco controle, sugerindo que as plantas selecionadas estão infectadas por haplótipos fracos de CTV, com potencial para serem utilizadas como plantas matrizes. A cross-protection program citrus tristeza disease, caused by Citrus tristeza virus (CTV) has been developed in the Parana State, to obtain Pera orange mother plants. Thus, 20 plants were selected in the orange commercial fields of the North and Northwest region. Comparisons between mild and severe recognized isolates were made. The results showed mild-to-moderate stem pitting symptoms and the great majority of the isolates grouped up with the mild control isolate. These results suggest that the plants selected were infected by CTV mild haplotypes showing that they have mother plant potential

Caracterização biológica e molecular de isolados do Citrus tristeza virus com potencial para utilização em programas de pré-imunização