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No Laboratório de Quarentena Vegetal da Embrapa Recursos Genéticos e Biotecnologia, foram realizadas análises fitossanitárias em tubérculos de batata, introduzidos no país para pesquisa, procedentes da França. Alguns desses tubérculos, em exame direto sob microscópio estereoscópio, apresentaram lesões deprimidas de aspecto encharcado, coloração marrom-arroxeada, casca amolecida e picnídios negros sobre as lesões. Em lâminas, sob microscópio de luz, foram observadas características morfológicas do fungo Phoma exigua var. foveata, praga exótica e de quarentena A1 para o Brasil. Para o teste de patogenicidade foram utilizados tubérculos sadios de batata inoculados com cultura pura do fungo isolado dos tubérculos sintomáticos. Sintomas de apodrecimento foram observados após 3 dias da inoculação, evoluindo até o 8º dia, quando o fungo foi re-isolado dessas lesões, em BDA, e confirmado o agente etiológico da doença. Os tubérculos apresentando sintomas foram incinerados e aqueles aparentemente sadios foram tratados com fungicida sistêmico, plantados em quarentenário por duas gerações e re-analisados. Após confirmação da sanidade dos tubérculos, os mesmos foram enviados ao requisitante. Potato tubers from France and introduced to Brazil for research purposes were subjected to phytosanitary analysis at the Quarantine Laboratory of Embrapa Genetic Resources and Biotechnology (Cenargen). Some of the tubers showed sunken, water-soaked, brownish-purple lesions, with soft skin, and black pycnidia were visible in the lesions using a stereomicroscope. Examination of the fungal structures on microscope slides using light microscopy demonstrated that the fungus had the morphology of Phoma exigua var. foveata, an A1 quarantine pest for Brazil. Pathogenicity tests were carried out inoculating healthy potato tubers with a pure culture of the fungus. Rot symptoms were observed 3 days after inoculation and progressed until 8 days. The fungus was re-isolated from the lesions on potato dextrose agar, confirming that the fungus was the etiological agent of the disease. The tubers that were symptomatic were incinerated, and the other healthy-looking tubers were treated with a systemic fungicide, cultivated under quarantine for two generations, and then reanalyzed. After confirming that they were free of the pathogen, the tubers were sent to their destination solicitor

Interceptação de Phoma exigua var. foveata, praga exótica e quarentenária para o Brasil, em germoplasma de batata procedente da França