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O Grapevine virus A (GVA) está associado à "Acanaladura do lenho de Kober", uma doença do complexo rugoso da videira (Vitis spp.). Neste trabalho, um isolado brasileiro de GVA (GVA-RS) foi caracterizado biologicamente por transmissão mecânica para cinco hospedeiras herbáceas e por enxertia na videira indicadora cv. Kober 5BB, e também por sorologia. O RNA total foi extraído de videira infetada cv. Pirovano 65. Para a RT-PCR, dois pares de oligonucleotídeos foram utilizados. Dois fragmentos de DNA, 430 e 451 pb, apresentando sobreposição parcial de nucleotídeos, foram amplificados por PCR. A seqüência do gene da proteína capsidial do GVA-RS com 597 nucleotídeos e 198 aminoácidos deduzidos, com massa molecular calculada de 21,6 kDa, foi alinhada a outros isolados virais. As seqüências de nucleotídeos e aminoácidos deduzidos do GVA-RS apresentaram maior identidade, 91,4% e 95,4%, respectivamente, com um isolado italiano. O GVA-RS apresentou expressiva divergência dos Vitivirus Heracleum latent virus (HLV), Grapevine virus B (GVB) e Grapevine virus D (GVD), com identidade de nucleotídeos variando de 76% a 83,1%. The Grapevine virus A (GVA) is associated with the Kober stem grooving disease, which is part of the grapevine (Vitis spp.) rugose wood complex. In this work, a Brazilian isolate of GVA (GVA-RS) was characterized biologically by mechanical transmission to five herbaceous hosts, by grafting on Kober 5BB grapevine indicator and by serology. Total RNA was extracted from infected grapevine cv Pirovano 65. For a reverse transcribed polymerase chain reaction, two sets of primers were used, resulting in two partially overlapping DNA fragments of 430 and 451 base pairs. The coat protein gene sequence of GVA-RS with 597 nucleotides and 198 deduced amino acids with a predicted Mr of 21.6 kDa, was aligned with those of other viral isolates. The nucleotide and the amino acid sequences of GVA-RS were closest in identity to an Italian isolate, 91.4% and 95.4%, respectively. The GVA-RS exhibited a strong divergence to the vitiviruses Vitivirus Heracleum latent virus (HLV), Grapevine virus B (GVB) and Grapevine virus D (GVD), displaying nucleotide identity of 76% - 83.1%

Detecção de um isolado de Grapevine virus A e caracterização do gene da proteína capsidial