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A hexa-oligosaccharide was obtained by partial acid hydrolysis from botryosphaeran, an exopolysaccharide (EPS) &#946;(1&#8594;3; 1&#8594;6)-D-glucan type, produced by the ascomyceteous fungus Botryosphaeria rhodina. The oligosaccharide was purified by gel filtration and charcoal-Celite column chromatography and the analysis was followed by HPAEC/PAD. The structure was determined by NMR spectroscopy and mass spectrometry, which showed that the oligosaccharide consists of six &#946;-D-glucopiranosyl units O-6 substituted (gentiohexaose).FAPESPCNPqFundação Araucári

Purificação e caracterização de uma gentioexaose obtida de botriosferana por hidrólise ácida parcial